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Monday, September 16, 2013

The Effects On Growth Lab

Introduction- There is many important factors that will affect the make up of microbes. These factors butt joint sometimes speed up or decompress down growth extremity, or even forego the process if the conditions are too extreme. These factors are temperature, pH, and osmotic pressure. I guess the temperature or the pH factor will pitch the nigh effect on the microbes in the experiment. Materials: Nutrient finishing fund of staphylococcusylococcus epidermidis, genus genus Serratia marcescen, and Bacillus megaterium. 2 Nutrient solid food agar Slants 5 vacuum tubes of nutrient broth An incubator at 25°C and 37°C and 55°C 5 petri plates 5 pipettes 1 tube of nutrient broth 1 bottle of nutrient agar pee baths at different temperatures Petri plates of agar 0.5% NaCl Petri plates of agar 5% NaCl Petri plates of agar 10% NaCl petri plates of milk salt agar 15% NaCl regularity The method can be located in the lab manual. We were to inocu late each one of our petri plates or our broth tubes or our tube slants with each one of the 3 cultures we were given. We then followed the directions in displace the different specimen in either the torrid urine baths, in the incubator or fridge banding at the specified temperatures to find the optimum growth of the cultures.
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Results -= no growth, +- = ok growth, ++ = good growth +++ = Think growth temperature Temp| 5°C| 28°C| 38°C| 55°C| staphylococcusBacillum| +--| ++-| ++++| +--| S. temp| 27°C| 38°C| | | Serritia Col-Pig-| -++-+++| -+++---| | | pH pH| 3| 7| 10| Staph| --| +-| ++| Serrati a| --| ++| +-| Bacillus| --| +-| +++| | ! | | | O.P.| 0.5%| 5%| 10%| Staph| +-| ++| ++| Serratia| ++| +++| --| Bacillus| +++| +++| ++-| | | | | Temp Lethar Temp| | 55°C| | 15 minute| 30 min| Staph| ++-| ++-| Serr ColPig| ++--| +--| Bacillus| +++| +++| watchword As we can see from the results of the lab, we can see thither are many variables which can help or oppress the...If you want to substantiate a full essay, order it on our website: OrderCustomPaper.com

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