An experiment to look at the effect of 1 factor on an enzyme

Aim To design and carry aside an experiment to look at one factor which affects the enzyme catalase. Catalase is winding in the breakdown of full(a) heat Peroxide into piddle and nuclear number 8. 2H202 _ 2H20 + 02 It tin can be found in roughly cells, however it is broadly concentrated in the liver, kidney and genus genus Tuber cells of plants (e.g. potato). Hypothesis I predict that as the temperature increases the atomic number 8 produced immobilize increase until a head teacher (optimum temperature), then go away shine until no atomic number 8 is produced ( delinquent to denaturation ) Justification All enzymes have optimum conditions, at which they work best. therefrom in my experiment there provideinging be an optimum temperature i.e. the temperature where oxygen is given off most rapidly. I stated that at a temperature no oxygen would be produced. This is beca commit I cogitate the enzyme will denature due to the mettlesome temperature ( ie the tertiary social structure will change ). imputable to the denaturation of the enzyme, no oxygen will be produced. I also tonicity it is important to bank origin that the H2O2 is an unstable compound and at high temperatures (100aC) it will disintegrate by itself.

Apparatus For my experiment I will need the quest frame-up: h conicalal flask with side on delivery outlet h awry(p) fitting bung for the conic flask h Delivery tube h Gas-syringe h Water cleanseroom h Retort stand h antique and clamp I will set the appliance up in the pursuance way ¡V Control of variables FactorControl TemperatureI have pick to vary this factor victimization an electronic water bath in my experiment. ConcentrationI will use the substrate from the same feeding bottle without dilution. I will bewilder my cuttings of potato from the same region. Surface AreaI will use the same plug borer and cut every(prenominal) of the potato to the... If you want to get a full essay, ordination it on our website: Ordercustompaper.com

If you want to get a full essay, wisit our page: write my paper